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Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, pre-cast protein gel, nucleic acid purification, PCR reagent...
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Biohelix/IsoAmp®III Universal tHDA Kit/H0120S/50 reactions (50 µl reaction)

Biohelix/IsoAmp®III Universal tHDA Kit/H0120S/50 reactions (50 µl reaction)
  • Biohelix/IsoAmp®III Universal tHDA Kit/H0120S/50 reactions (50 µl reaction)
商品介绍

Description:

  

IsoAmpIIIUniversaltHDAkitcanbeusedtoamplifyanddetectshortDNAsequences(70bp-120bp)ataconstanttemperature.Thekitcanbeusedwithavarietyoftemplates,includingmicrobialgenomicDNA,viralDNA,plasmidDNA,andCDNA.AsinglecopyoftargetDNAcanbeamplifiedbytHDAanddetectedbyagarosegelelectrophoresiswhenoptimizedprimersandbufferareused.

IsoAmp®IIIUniversaltHDAkitisbasedonathird-generationtHDAformulaformorerobustandspecificamplificationwithreducednon-specificproductsduetoprimer-dimer.Insomecases,rapidamplificationmaybeachievedbyaddingmoreIsoAmp®IIIEnzymeMixintothereaction(unpublishedobservation).ThevolumeofIsoAmp®IIIEnzymeMixhasbeenreducedto2µLperreaction,whichallowstheusertoaddmoreenzymes(2Xor3X)forpossIBLehigh-speedamplification.IsoAmp®IIIEnzymeMix(Cat#H1020S)canbepurchasedseparately.

IsoAmp®IIIUniversaltHDAkitsupportsallreactionswhicharesupportedbyIsoAmp®IIUniversaltHDAkit.Selectedexamplesareincludedinthismanual,includingend-pointtHDAandreal-timequantitativeHDA(qHDA)inbothone-stepandtwo-stepformats.AControlTemplateandasetofspecificprimersaresuppliedforthepositivecontrolreaction.

 

  


Figure1.HDATechnology.

  

 


Figure2.
PositivecontrolassayswithvaryingamountofControlTemplate.
Positivecontrolassayswereconductedfollowingtheone-stepqHDAprotocol.ControlTemplatewasusedat5ng-500fgperassayintriplicate.

*Negativecontrolreactionsmayproduceprimer-dimer,whichcanbedistingushedfromspecificampliconsbygel-analysisorbymelting-curveanalysis.

 

Applications:

  


UsedtoamplifyanddetectshortDNAsequences(80-120bp)

-downloadManual

Advantages:
UseofhelicasetoseparateDNAeliminatesneedforThermocycler
Reactionscanbeperformedatconstanttemperature.
Workswithavarietyoftemplates,includingbacterialgenomicDNA,viralDNA,plasmidDNAandcDNA.
AslowasasinglecopyoftargetDNAcanbeamplifiedunderoptimizedconditions.
Canbeusedforreal-timequantitativeor/andreversetranscriptionHDA.

   

KitComponents:

  

IsoAmp®IIIEnzymeMix(50reactions)
IsoAmp®dNTPSolution(50reactions)
10XAnnealingBufferII
MgSO4(100mM)
NaCl(500mM)
pCNG1(ControlTemplate:1ng/µl)
NGF3(ForwardPrimer:5µM)
NGR3(ReversePrimer:5µM)
     

StorageConditions:

  

StorageTemperature:
-20ºC

RecommendedstorageconditionsforthetHDAkitare-20°Cforshort-term(lessthan6months)storageand-80°Cforstoragegreaterthan6months.     

QualityControl:

 


QualityAssuranceStatement:

KitcomponentswereindividuallytestedforoptimalperformanceintHDA.

     

Legal:

 
Licenses/Patents/Disclaimers:
NOTICETOPURCHASER:ThisproductiscoveredbyU.S.patents7,282,328and7,662,594andpendingforeignpatents.Thepurchasepriceofthisproductincludesalimited,non-transferablelicenseforresearchuseonlyunderthepatentsownedbyBiohelix.Nootherlicenseunderthesepatentsisconveyedexpresslyorbyimplicationtothepurchaserbythepurchaseoftheproduct.TheProductisnottobeusedfordiagnosticpurposesnorisitintendedforhumanuse.Thisproductmaynotberesold,modifiedforresale,orusedtomanufacturecommercialproductswithoutwrittenapprovalofBioHelixCorp.FurtherinformationregardingthisproductmaybeobtainedbycontactingBioHelixatinformation@biohelix.com.

   
品牌介绍

Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Our products, to name a few, include DNA markers, protein markers, pre-cast protein gel, nucleic acid purification, PCR reagents and so on. Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, GeneDirex, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.

With the expansion of product lines as well as customers, we discover a greater need of good quality supply of bio-reagents from medical circle. Mostly, these needs are for diagnostic products. The demand of good quality bio-reagents for diagnostic devices opens a whole new possibility of innovation and motivation for Bio-Helix.

We have a vision that one day our life span can be elongated; life quality can be improved by early detection and subsequent proper treatment of all diseases. Ancient Chinese believed that a good doctor treats disease before illness, instead of after its onset. In Bio-Helix, a new brand, LifeDirex, is created to fulfill this vision. LifeDirex will be focusing on supplying the best and most innovative reagents for diagnostic tools to promote earliest detection of diseases as possible.

Our mission is to provide the best quality products and to develop novel technologies that assist researchers to find solutions in quest of scientific achievements. Furthermore, we decide now is the best time to enter the market of in vitro diagnostic device (IVDD). It is our goal to become the best solution provider for both research laboratory and IVDD.

Driven by desires to improve life quality and achieve scientific excellence, we operate with the highest standards of integrity. We also extend the spirit to our services and global value to our customers all around the world. A diverse workforce and network of collaboration form a great mix of company culture. Bio-Helix is not only a company of equal opportunity but also works hard to provide a friendly and growing environment.


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